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A sputum culture is
a test to find bacteria or
fungi (plural of fungus) that are infecting the lungs
or airways. Sputum is a thick fluid made in the lungs and in the
airways leading to the lungs. A sample of sputum is placed in a container with
substances that cause the growth of bacteria or fungi. If no bacteria or
fungi grow, the culture is negative. If organisms that can cause infection
grow, the culture is positive. The type of bacterium or
fungus will be found with a microscope or by chemical tests.
If bacteria or fungi that can cause infection grow in the culture, other
tests may be done. These tests help to find which antibiotic will work best to
treat the infection. This is called susceptibility or
You may be asked to cough to provide the sputum sample. Some people can't
cough deeply enough to produce a sample. They can breathe in a special mist to
help them cough.
A sputum culture is done to:
Do not use mouthwash before you collect your
sputum sample. Some types of mouthwash can kill bacteria and could affect
bronchoscopy will be used to collect your sputum
sample, your doctor will tell you how soon before the test to stop eating and drinking. Follow the instructions exactly about when to
stop eating and drinking. If you don't, your surgery may be canceled. If your doctor told you to take your medicines on the day of surgery, take them with
only a sip of water.
Tell your doctor
if you have recently taken antibiotics.
In most cases, the sputum sample is collected
early in the morning before you eat or drink anything. In some cases, three or more morning samples may be
needed. (This is often done if you may have tuberculosis.)
If you wear dentures, you will need to take them out before
you collect your sputum sample. Then rinse your mouth with water. Next, take a deep
breath and cough deeply to get a sample of sputum. The person taking the sample may tap on your chest. This tapping helps loosen the
sputum in your lungs before you cough. If you still have trouble coughing up a
sample, you may be asked to inhale an aerosol mist to help you cough.
Some people may need bronchoscopy to collect a sputum sample. A thin, lighted tube (bronchoscope) is put through your
mouth or nose into the airways leading to your lungs. You will get
medicine that numbs your throat and nose so you do not feel pain from
the bronchoscope. You may also get a sedative to make you sleepy during
the test. To collect the sample, a salt solution may be washed into
the airway and then suctioned into a container. A small, thin brush may be used
to collect a sample.
Suction can also be used to collect a sputum sample. A soft, flexible tube (called a nasotracheal
catheter) is put through the nose and down the throat. Suction is applied
for up to 15 seconds to collect the sample. This method is often used for people who are very sick or unconscious.
sputum sample will be placed in a container with a growth medium or culture medium. These are substances that help with the growth of bacteria or fungi. Bacteria usually need 2 to 3 days to grow. Fungus often takes a week or longer to grow. The organism that causes
tuberculosis may take 6 weeks to grow. Any bacteria or fungi that grow will be
found under a microscope or by chemical tests. Sensitivity testing, to
find the best antibiotic to use, often
takes 1 to 2 more days.
If you have some pain when you take a deep
breath or when you cough, giving a sputum sample may be uncomfortable. If you
need to inhale the aerosol mist to produce a sample, you will likely feel
a strong urge to cough.
During bronchoscopy or
collection of a sputum sample using a catheter, you may feel a strong urge to
cough. This can happen as the bronchoscope or catheter passes into the back of your throat. You
may also feel as if you can't breathe. Try to relax. Breathe slowly.
If you are given medicine to numb
your throat and nose, you may feel as if your tongue and throat are swollen. You may find it hard to swallow.
Your throat may feel sore after bronchoscopy
or collection of a sputum sample using a nasotracheal catheter.
you have severe
bronchitis, you may find it hard to breathe
during collection of a sputum sample using a nasotracheal catheter.
A sputum culture is a test to find bacteria or
fungi that are infecting the lungs or airways. Some types of bacteria or fungi grow quickly in a culture, and some
grow slowly. Test results may take from 1 day to several weeks. How long your results take depends on
the type of infection your doctor thinks you may have. Some organisms do not grow in a
standard culture and need a special growth medium to be found in a sputum
culture. (Examples are Chlamydophila pneumoniae and mycoplasma.)
Sputum that has passed through
the mouth normally contains several types of harmless bacteria. These include some
types of strep (Streptococcus) and staph (Staphylococcus). The culture should
not show any harmful bacteria or fungi. Normal culture results are
Harmful bacteria or fungi are
present. The most common harmful bacteria in a sputum culture are those that
tuberculosis. If harmful bacteria or fungi grow, the culture
If test results point to an infection, sensitivity testing
may be done. This testing helps to find the best antibiotic to kill the bacteria or fungus.
You may not be able to have the test, or the results may not be helpful, if:
Other Works Consulted
Chernecky CC, Berger BJ (2008). Laboratory Tests and Diagnostic Procedures, 5th ed. St. Louis:
Fischbach FT, Dunning MB III, eds. (2009).
Manual of Laboratory and Diagnostic Tests, 8th ed.
Philadelphia: Lippincott Williams and Wilkins.
Pagana KD, Pagana TJ (2010). Mosby’s Manual of Diagnostic and Laboratory Tests, 4th ed. St. Louis: Mosby.
ByHealthwise StaffPrimary Medical ReviewerAdam Husney, MD - Family MedicineSpecialist Medical ReviewerRobert L. Cowie, MB, FCP(SA), MD, MSc, MFOM - Pulmonology
Current as ofFebruary 12, 2015
Current as of:
February 12, 2015
Adam Husney, MD - Family Medicine & Robert L. Cowie, MB, FCP(SA), MD, MSc, MFOM - Pulmonology
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